1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Use at an assay dependent dilution.
Application notesIs unsuitable for IHC-P or IP.
FunctionEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
PathwayProtein modification; protein ubiquitination.
Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Skp1 antibody [EPR3304] (ab76502)
All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution
Lane 1 : 293T cell lysate Lane 2 : HeLa cell lysate Lane 3 : PC-12 cell lysate Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP labeled IgG at 1/2000 dilution
Predicted band size : 19 kDa Observed band size : 19 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Skp1 antibody [EPR3304] (ab76502)This image is courtesy of an anonymous Abreview.
ab76502 staining Skp1 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol, blocked with 1% BSA for 30 minutes at 24°C then incubated with ab76502 at a 1/500 dilution for 1 hour at 24°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
References for Anti-Skp1 antibody [EPR3304] (ab76502)
This product has been referenced in:
Kainulainen M et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol88:3464-73 (2014).
Read more (PubMed: 24403578) »