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A synthetic peptide corresponding to a sequence at the N terminus of human SKP2, differing from the related rat and mouse sequence by three amino acids.
Our Abpromise guarantee covers the use of ab68455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|ICC||Use a concentration of 1 - 2 µg/ml. Acetone fixed cells. Antigen retrieval by Pepsin and Trypsin is required.|
ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.