Overview

  • Product nameAnti-SKP2 antibody
    See all SKP2 primary antibodies
  • Description
    Rabbit polyclonal to SKP2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide raised within aa: 1-50 of human SKP2.

Properties

Applications

Our Abpromise guarantee covers the use of ab87809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at 10-15 µg/mg of lysate.

Target

  • FunctionSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK2/Cyclin A associated protein p45 antibody
    • Cyclin A/CDK2 associated protein p45 antibody
    • Cyclin-A/CDK2-associated protein p45 antibody
    • F box protein Skp2 antibody
    • F box/LRR repeat protein 1 antibody
    • F-box protein Skp2 antibody
    • F-box/LRR-repeat protein 1 antibody
    • FBL 1 antibody
    • FBL1 antibody
    • FBXL 1 antibody
    • FBXL1 antibody
    • FLB 1 antibody
    • FLB1 antibody
    • MGC1366 antibody
    • p45 antibody
    • p45skp2 antibody
    • S phase kinase associated protein 2 (p45) antibody
    • S phase kinase associated protein 2 antibody
    • S-phase kinase-associated protein 2 antibody
    • S-phase kinase-associated protein 2 E3 ubiquitin protein ligase antibody
    • SKP 2 antibody
    • Skp2 antibody
    • SKP2_HUMAN antibody
    see all

Anti-SKP2 antibody images

  • Lane 1: ab87809 (1µg/ml) immunoprecipitating SKP2 in HeLa Whole Cell Lysate (1mg for IP, 20% loaded).
    Lane 2: positive control IP (done using an antibody that recognises downstream epitope).
    Lane 3: Control IgG
  • All lanes : Anti-SKP2 antibody (ab87809) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg


    Predicted band size : 48 kDa

References for Anti-SKP2 antibody (ab87809)

ab87809 has not yet been referenced specifically in any publications.

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The anti-SKP2 antibody (ab87809) has been used with HeLa whole cell lysate in order to detect the SKP2 protein. If your customer would like to purchase this as a pre-prepared lysate this can be purchased under the...

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