Overview

  • Product nameAnti-SLAM / CD150 antibody [IPO-3]
    See all SLAM / CD150 primary antibodies
  • Description
    Mouse monoclonal [IPO-3] to SLAM / CD150
  • SpecificityThis antibody is specific for SLAM.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Fr, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Activated B cell line

  • EpitopeThe exact epitope recognised by this antibody has not been mapped.
  • General notes


    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS pH 7.2, 0.01% sodium azide
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A purified from ascetic fluid
  • Primary antibody notesA novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
  • ClonalityMonoclonal
  • Clone numberIPO-3
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21379338
ELISA 1/750.
IHC-Fr 1/100.
Flow Cyt 1/750.
IP Use at an assay dependent concentration.

Target

  • FunctionHigh-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
  • Tissue specificityConstitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainThe most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
  • Post-translational
    modifications
    Phosphorylated by FYN.
  • Cellular localizationCell membrane. Present on the surface of B-cells and T-cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933415F16 antibody
    • AA177906 antibody
    • CD 150 antibody
    • CD150 antibody
    • CD150 antigen antibody
    • CDw150 antibody
    • Estm51 antibody
    • Ipo 3 antibody
    • IPO-3 antibody
    • Ipo3 antibody
    • MGC151472 antibody
    • MGC151476 antibody
    • OTTHUMP00000025670 antibody
    • OTTHUMP00000060252 antibody
    • RGD1560634 antibody
    • Signaling lymphocytic activation molecule antibody
    • Signaling lymphocytic activation molecule family member 1 antibody
    • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
    • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
    • Signaling lymphocytic activation molecule precursor antibody
    • Slaf1 antibody
    • SLAF1_HUMAN antibody
    • SLAM family, member 1 antibody
    • Slamf 1 antibody
    • SLAMF1 antibody
    • SLAMF1 protein antibody
    see all

Anti-SLAM / CD150 antibody [IPO-3] images

  • ab2604, staining SLAM / CD150 in Raji cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100 before being incubated with primary antibody. A fluorescence-conjugated anti-mouse IgG was used as the secondary antibody.

References for Anti-SLAM / CD150 antibody [IPO-3] (ab2604)

This product has been referenced in:
  • Avota E  et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . Read more (PubMed: 21379338) »
  • Noyce RS  et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). Read more (PubMed: 21901103) »

See all 2 Publications for this product

Product Wall



You are correct the calculated molecular weight of SLAM is ca 37 kDa.

Modification can significantly increase the molecular weight of proteins. SLAM is a sialylated phosphoglycoprotein.

The effect the glycosylation has on ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"