• Product name
  • Description
    Rabbit polyclonal to SLC12A1
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, African green monkey
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cells; Kidney tissue.



Our Abpromise guarantee covers the use of ab60301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.


  • Function
    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificity
    Kidney specific.
  • Involvement in disease
    Defects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BSC1 antibody
    • Bumetanide sensitive sodium 3 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
    • Kidney specific Na K Cl symporter antibody
    • Kidney-specific Na-K-Cl symporter antibody
    • MGC48843 antibody
    • Na K 2Cl cotransporter antibody
    • NKCC2 antibody
    • potassiumchloride cotransporter 2 antibody
    • S12A1_HUMAN antibody
    • Slc12a1 antibody
    • sodium potassium chloride cotransporter 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
    • Solute carrier family 12 member 1 antibody
    see all

Anti-SLC12A1 antibody images

  • All lanes : Anti-SLC12A1 antibody (ab60301) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Immunohistchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC12A1 with ab60301 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-SLC12A1 antibody (ab60301)

ab60301 has not yet been referenced specifically in any publications.

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