• Product nameAnti-SLC12A1 antibody
    See all SLC12A1 primary antibodies
  • Description
    Mouse monoclonal to SLC12A1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Recombinant fragment: AKTDASFHAY DSHTNTYYLQ TFGHNTMDAV PKIEYYRNTG SISGPKVNRP SLLEIHEQLA KNVAVTPSSA DRVANGDGIP GDEQAENKED DQA, corresponding to amino acids 80-172 of Human SLC12A1 with a 26kDa tag.

  • Positive control
    • SLC12A1 transfected 293T cell lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab77320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 121 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionElectrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Tissue specificityKidney specific.
  • Involvement in diseaseDefects in SLC12A1 are the cause of Bartter syndrome type 1 (BS1) [MIM:601678]. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS1 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS1 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.
  • Sequence similaritiesBelongs to the SLC12A transporter family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSC1 antibody
    • Bumetanide sensitive sodium 3 antibody
    • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2 antibody
    • Kidney specific Na K Cl symporter antibody
    • Kidney-specific Na-K-Cl symporter antibody
    • MGC48843 antibody
    • Na K 2Cl cotransporter antibody
    • NKCC2 antibody
    • potassiumchloride cotransporter 2 antibody
    • S12A1_HUMAN antibody
    • Slc12a1 antibody
    • sodium potassium chloride cotransporter 2 antibody
    • solute carrier family 12 (sodium/potassium/chloride transporters) antibody
    • Solute carrier family 12 member 1 antibody
    see all

Anti-SLC12A1 antibody images

  • Anti-SLC12A1 antibody (ab77320) at 5 µg/ml + immunogen at 0.2 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 121 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SLC12A1 antibody (ab77320) at 5 µg/ml

    Lane 1 : SLC12A1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 121 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References for Anti-SLC12A1 antibody (ab77320)

ab77320 has not yet been referenced specifically in any publications.

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