Overview

  • Product name
  • Description
    Goat polyclonal to SLC12A4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-DPSHAPDNFRE

    , corresponding to internal sequence amino acids 995-1005 of Human SLC12A4 (NP_005063.1).

  • Positive control
    • Human Heart and Muscle lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77340 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 121 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity.
    • Tissue specificity
      Ubiquitous. Levels are much higher in erythrocytes from patients with Hb SC and Hb SS compared to normal AA erythrocytes. This may contribute to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of HbS. Isoform 1 was not detected in circulating reticulocytes.
    • Sequence similarities
      Belongs to the SLC12A transporter family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Electroneutral potassium chloride cotransporter 1 antibody
      • Electroneutral potassium-chloride cotransporter 1 antibody
      • Erythroid K Cl cotransporter 1 antibody
      • Erythroid K-Cl cotransporter 1 antibody
      • FLJ40489 antibody
      • hKCC 1 antibody
      • hKCC1 antibody
      • KCC 1 antibody
      • KCC1 antibody
      • Potassium transport protein antibody
      • Potassium/chloride cotransporter 1 antibody
      • S12A4_HUMAN antibody
      • Slc12a4 antibody
      • Solute carrier family 12 (potassium/chloride transporters) member 4 antibody
      • Solute carrier family 12 member 4 antibody
      see all

    Anti-SLC12A4 antibody images

    References for Anti-SLC12A4 antibody (ab77340)

    ab77340 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77340.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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