Overview

  • Product name
  • Description
    Goat polyclonal to SLC12A6
  • Specificity
    ab77338 is expected to recognise all 6 reported isofoms (NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide:

    SRHIDVCSKTKE

    , corresponding to internal sequence amino acids 384-395 of Human SLC12A6 (NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1; NP_005126.1; NP_598408.1.)

  • Positive control
    • Human Heart and kidney lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77338 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
    • Tissue specificity
      Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
    • Involvement in disease
      Agenesis of the corpus callosum, with peripheral neuropathy
    • Sequence similarities
      Belongs to the SLC12A transporter family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Basolateral cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACCPN antibody
      • Electroneutral potassium-chloride cotransporter 3 antibody
      • Furosemide sensitive KCl cotransporter 3 antibody
      • Gaxp antibody
      • K-Cl cotransporter 3 antibody
      • KCC 3 antibody
      • KCC 3A antibody
      • KCC 3B antibody
      • KCC3 A antibody
      • KCC3 antibody
      • KCC3 B antibody
      • KCC3A antibody
      • KCC3B antibody
      • Potassium chloride cotransporter 3 antibody
      • Potassium chloride cotransporter KCC3a S3 antibody
      • S12A6_HUMAN antibody
      • SLC12 A6 antibody
      • SLC12A 6 antibody
      • SLC12A6 antibody
      • Solute carrier family 12 (potassium/chloride transporters), member 6 antibody
      • Solute carrier family 12 member 6 antibody
      • Solute carrier family 12, member 6 antibody
      see all

    Images

    • Anti-SLC12A6 antibody (ab77338) at 0.1 µg/ml + Human Heart lysate (in RIPA buffer) at 35 µg

      Predicted band size : 128 kDa
      Observed band size : 128 kDa

    References

    ab77338 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77338.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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