The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
Tissue specificityHighly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
Involvement in diseaseAgenesis of the corpus callosum, with peripheral neuropathy
Sequence similaritiesBelongs to the SLC12A transporter family.