The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
Involvement in disease
Agenesis of the corpus callosum, with peripheral neuropathy