• Product nameAnti-SLC12A6 antibody
    See all SLC12A6 primary antibodies
  • Description
    Rabbit polyclonal to SLC12A6
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human SLC12A6.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Kidney; Skeletal Muscle.



Our Abpromise guarantee covers the use of ab92951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).


  • FunctionMediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.
  • Tissue specificityHighly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney (at protein level).
  • Involvement in diseaseAgenesis of the corpus callosum, with peripheral neuropathy
  • Sequence similaritiesBelongs to the SLC12A transporter family.
  • Post-translational
  • Cellular localizationBasolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACCPN antibody
    • Electroneutral potassium-chloride cotransporter 3 antibody
    • Furosemide sensitive KCl cotransporter 3 antibody
    • Gaxp antibody
    • K-Cl cotransporter 3 antibody
    • KCC 3 antibody
    • KCC 3A antibody
    • KCC 3B antibody
    • KCC3 A antibody
    • KCC3 antibody
    • KCC3 B antibody
    • KCC3A antibody
    • KCC3B antibody
    • Potassium chloride cotransporter 3 antibody
    • Potassium chloride cotransporter KCC3a S3 antibody
    • S12A6_HUMAN antibody
    • SLC12 A6 antibody
    • SLC12A 6 antibody
    • SLC12A6 antibody
    • Solute carrier family 12 (potassium/chloride transporters), member 6 antibody
    • Solute carrier family 12 member 6 antibody
    • Solute carrier family 12, member 6 antibody
    see all

Anti-SLC12A6 antibody images

  • All lanes : Anti-SLC12A6 antibody (ab92951) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 128 kDa
    Observed band size : 122,128 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes
  • ICC/IF image of ab92951 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92951 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa cells at 1ug/ml, and in Methanol fixed (100%, 5min) HepG2 cells at 1ug/ml

References for Anti-SLC12A6 antibody (ab92951)

ab92951 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab92951.
Please use the links above to contact us or submit feedback about this product.