Overview

  • Product name
    Anti-SLC12A8 antibody
  • Description
    Rabbit polyclonal to SLC12A8
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 433-577 of Human SLC12A8.

  • Positive control
    • RT-4 and U-251 MG cell lysate; Human plasma; Human duodenum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cation/chloride cotransporter that may play a role in the control of keratinocyte proliferation.
  • Tissue specificity
    Ubiquitous with very low level in normal skin.
  • Involvement in disease
    Note=SLC12A8 has been identified as a possible susceptibility gene for psoriasis mapped to chromosome 3q21 (PSORS5).
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cation-chloride cotransporter 9 antibody
    • CCC9 antibody
    • DKFZp686L18248 antibody
    • FLJ23188 antibody
    • OTTHUMP00000215536 antibody
    • OTTHUMP00000215625 antibody
    • S12A8_HUMAN antibody
    • Slc12a8 antibody
    • Solute carrier family 12 (potassium/chloride transporters), member 8 antibody
    • Solute carrier family 12 member 8 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122152 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-SLC12A8 antibody (ab122152) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab122152, at 1/25, staining SLC12A8 in paraffin embedded Human duodenum by Immunohistochemistry.

References

ab122152 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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