Overview

  • Product name
    Anti-SLC13A3 antibody
  • Description
    Rabbit polyclonal to SLC13A3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: LYGGLSFRGW RKNKSEIRTN AEDRARAVIR EEYQNLGPIK FAEQAVFILF, corresponding to middle sequence amino acids 253-302 of Human SLC13A3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate and human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab56017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using a peptide based assay:1/312500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    High-affinity sodium-dicarboxylate cotransporter that accepts a range of substrates with 4-5 carbon atoms. The stoichiometry is probably 3 Na(+) for 1 divalent succinate.
  • Tissue specificity
    Expression is highest in kidney. Detected in placenta, brain, liver and pancreas.
  • Sequence similarities
    Belongs to the SLC13A transporter (TC 2.A.47) family. NADC subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • +dicarboxylate cotransporter 3 antibody
    • H sapiens mRNA for hepatocyte nuclear factor 4 gamma antibody
    • hNaDC3 antibody
    • Na antibody
    • Na(+)/dicarboxylate cotransporter 3 antibody
    • NaDC 3 antibody
    • NaDC-3 antibody
    • NADC3 antibody
    • S13A3_HUMAN antibody
    • SDCT 2 antibody
    • SDCT2 antibody
    • SLC13A3 antibody
    • Sodium dependent high affinity dicarboxylate transporter 2 antibody
    • Sodium dependent high affinity dicarboxylate transporter 3 antibody
    • Sodium-dependent high-affinity dicarboxylate transporter 2 antibody
    • Solute carrier family 13 (sodium dependent dicarboxylate transporter), member 3 antibody
    • Solute carrier family 13 member 3 antibody
    see all

Images

  • Western blot - Anti-SLC13A3 antibody (ab56017)
    Western blot - Anti-SLC13A3 antibody (ab56017)
    Anti-SLC13A3 antibody (ab56017) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 61 kDa
    Observed band size: 54 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC13A3 antibody (ab56017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC13A3 antibody (ab56017)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC13A3 at 4-8µg/ml.

    Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References

This product has been referenced in:
  • Ju W  et al. Renal gene and protein expression signatures for prediction of kidney disease progression. Am J Pathol 174:2073-85 (2009). IHC-P ; Human, Mouse . Read more (PubMed: 19465643) »

See 1 Publication for this product

Publishing research using ab56017? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

lot GR72437
datasheet says 100 ug at 2 mg/ml
but received 100 ul

Thank you for your patience.
Yes, I can confirm that the concentration of the antibody is 1 mg/ml. We will update the datasheet accordingly to reflect this information.
I apologize for this mistake on the datasheet and the confusion caused. <...

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lot GR72437
datasheet says 100 ug at 2 mg/ml
but received 100 ul

Thank you for contacting us.
I have checked internally and presumably the correct concentration of the product is 1 mg/ml. I will doublecheck this with a couple of my colleagues on Monday (as they were out today), and will confirm this then.
...

Read More

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