Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC15A2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 432-481 (GNENNSLLIE SIKSFQKTPH YSKLHLKTKS QDFHFHLKYH NLSLYTEHSV) of human SLC15A2 (NP_066568).

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides.
  • Sequence similarities
    Belongs to the PTR2/POT transporter (TC 2.A.17) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ33407 antibody
    • Hydrogen ion/peptide cotransporter, kidney antibody
    • Kidney H(+)/peptide cotransporter antibody
    • kidney isoform antibody
    • Oligopeptide transporter antibody
    • Oligopeptide transporter kidney isoform antibody
    • PEPT2 antibody
    • Peptide transporter 2 antibody
    • S15A2_HUMAN antibody
    • Slc15a2 antibody
    • Solute carrier family 15 (H+/peptide transporter) member 2 antibody
    • solute carrier family 15 (oligopeptide transporter), member 2 antibody
    • Solute carrier family 15 member 2 antibody
    see all

Images

  • Anti-SLC15A2 antibody (ab83771) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab83771 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83771, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab83771 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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