Anti-SLC16A10 antibody - C-terminal (ab171649)

Overview

  • Product name
    Anti-SLC16A10 antibody - C-terminal
  • Description
    Rabbit polyclonal to SLC16A10 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC16A10 aa 300-329 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. (NP_061063.2).
    Database link: Q8TF71

  • Positive control
    • K562 cell line lysate; Human skeletal muscle tissue; K562 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171649 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 55 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Sodium-independent transporter that mediates the update of aromatic acid. Can function as a net efflux pathway for aromatic amino acids in the basosolateral epithelial cells.
  • Tissue specificity
    Strongly expressed in kidney and skeletal muscle and at lower level in placenta and heart.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • Post-translational
    modifications
    Not N-glycosylated.
  • Cellular localization
    Cell membrane. Basolateral cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MCT10 antibody
    • Aromatic amino acid transporter 1 antibody
    • MCT 10 antibody
    • Monocarboxylate transporter 10 antibody
    • MOT10_HUMAN antibody
    • SLC16A10 antibody
    • Solute carrier family 16 member 10 antibody
    • T-type amino acid transporter 1 antibody
    • TAT1 antibody
    see all

Images

  • Anti-SLC16A10 antibody - C-terminal (ab171649) at 1/100 dilution + K562 cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling SLC16A10 with ab171649 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometric analysis of K562 cells with ab171649 antibody at a dilution of 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated Donkey-anti-Rabbit secondary antibodies were used for the analysis.

References

ab171649 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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