Overview

  • Product nameAnti-SLC16A3 antibody
    See all SLC16A3 primary antibodies
  • Description
    Rabbit polyclonal to SLC16A3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from internal region of human SLC16A3.

  • Positive control
    • Extracts from LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab74109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
ELISA 1/40000.

Target

  • FunctionProton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • Tissue specificityHighly expressed in skeletal muscle.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • FormLactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family 16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains.
  • Alternative names
    • MCT 4 antibody
    • MCT3 antibody
    • MCT4 antibody
    • Monocarboxylate transporter 3 antibody
    • Monocarboxylate transporter 4 antibody
    • MOT4_HUMAN antibody
    • SLC16A3 antibody
    • Solute carrier family 16 member 3 antibody
    see all

Anti-SLC16A3 antibody images

  • All lanes : Anti-SLC16A3 antibody (ab74109) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 49 kDa
    Observed band size : 49 kDa

References for Anti-SLC16A3 antibody (ab74109)

This product has been referenced in:
  • Ghorbel MT  et al. Changes in renal medulla gene expression in a pre-clinical model of post cardiopulmonary bypass acute kidney injury. BMC Genomics 15:916 (2014). Read more (PubMed: 25331815) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% 37.5:1)
Sample Pig Tissue lysate - whole (Skeletal Muscle)
Specification Skeletal Muscle
Blocking step Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
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Verified customer

Submitted Dec 10 2013

Thank you for providing that information. It allows me to more fully understand the results that you have been observing.

There are a few further things I would like to clarify:

You mentioned that the anti-MCT1 has also been proving...

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