Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC17A5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 9-242 of Human SLC17A5.

  • Positive control
    • Jurkat whole cell lysate; HepG2 cells; Human BT474 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54, 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva.
  • Tissue specificity
    Found in fetal lung and small intestine, and at lower level in fetal skin and muscle. In the adult, detected in placenta, kidney and pancreas. Abundant in the endothelial cells of tumors from ovary, colon, breast and lung, but is not detected in endothelial cells from the corresponding normal tissues.
  • Involvement in disease
    Salla disease
    Infantile sialic acid storage disorder
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.
  • Cellular localization
    Cell membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AST antibody
    • H(+)/nitrate cotransporter antibody
    • H(+)/sialic acid cotransporter antibody
    • ISSD antibody
    • Membrane glycoprotein HP59 antibody
    • NSD antibody
    • S17A5_HUMAN antibody
    • SD antibody
    • Sialic acid storage disease antibody
    • Sialin antibody
    • SIASD antibody
    • Slc17a5 antibody
    • SLD antibody
    • Solute carrier family 17 (anion/sugar transportermember 5 antibody
    • Solute carrier family 17 member 5 antibody
    • Vesicular H(+)/Aspartate-glutamate cotransporter antibody
    see all

Images

  • Anti-SLC17A5 antibody (ab153920) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 54, 31 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded BT474 xenograft labeling SLC17A5 with ab153920 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed HepG2 cells labeling SLC17A5 with ab153920 at 1/500 dilution (green). Blue: Hoechst 33343 staining.

References

ab153920 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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