Anti-SLC20A2/PIT2 antibody (ab155259)
Key features and details
- Rabbit polyclonal to SLC20A2/PIT2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC20A2/PIT2 antibody
See all SLC20A2/PIT2 primary antibodies -
Description
Rabbit polyclonal to SLC20A2/PIT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat -
Immunogen
Recombinant fragment corresponding to Human SLC20A2/PIT2 aa 100-450 (internal sequence).
Database link: Q08357 -
Positive control
- 293T, A431, H1299 and Raji cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case. |
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case. |
Target
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Function
Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 493788 Cat
- Entrez Gene: 6575 Human
- Entrez Gene: 20516 Mouse
- Entrez Gene: 29502 Rat
- Omim: 158378 Human
- SwissProt: Q95L97 Cat
- SwissProt: Q08357 Human
- SwissProt: Q80UP8 Mouse
see all -
Alternative names
- Gibbon ape leukemia virus receptor 2 antibody
- Gibbon leukemia virus receptor 2 antibody
- GLVR 2 antibody
see all
Images
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Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution + Unboiled U87-MG cell lysate at 30 µg
Predicted band size: 70 kDa -
All lanes : Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution
Lane 1 : A431 whole cell extracts
Lane 2 : Unboiled A431 whole cell extracts
Predicted band size: 70 kDa -
Anti-SLC20A2/PIT2 antibody (ab155259) at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155259 has been referenced in 1 publication.
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927