Anti-SLC22A1 antibody (ab123128)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-SLC22A1 antibody
See all SLC22A1 primary antibodies -
DescriptionRabbit polyclonal to SLC22A1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 510-539 of Human SLC22A1 (O15245, NP_003048.1, NP_694857.1).
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Positive control
- HepG2 cell line lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab123128 is purified through a protein A column, followed by peptide affinity purification.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab123128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 61 kDa. |
Target
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FunctionTranslocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.
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Tissue specificityWidely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines.
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Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
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Post-translational
modificationsPhosphorylated. -
Cellular localizationBasolateral cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 6580 Human
- Omim: 602607 Human
- SwissProt: O15245 Human
- SwissProt: O15395 Human
- Unigene: 117367 Human
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Alternative names
- hOCT1 antibody
- OCT1 antibody
- oct1_cds antibody
see all
Images
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Western blot - Anti-SLC22A1 antibody (ab123128)Anti-SLC22A1 antibody (ab123128) at 1/100 dilution + HepG2 cell line lysates at 35 µg
Predicted band size: 61 kDa
Protocols
References
This product has been referenced in:
- Hubeny A et al. Expression of Organic Anion Transporting Polypeptide 1A2 in Red Blood Cells and Its Potential Impact on Antimalarial Therapy. Drug Metab Dispos 44:1562-8 (2016). Read more (PubMed: 27504015) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"