The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionProbably transports organic cations (By similarity). Appears not to be the agmatine transporter.
Tissue specificityExpressed at highest levels in kidney and brain. Expressed at high levels in skeletal muscle, heart, liver, placenta and white blood cells. Expressed at moderate levels in lung and spleen. Expressed at low levels in thymus, small intestine and colon. Expressed in several intestinal tumor cell lines.
Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
Developmental stageExpressed at low levels at 20-25 weeks of gestation in fetal brain, lung, liver and kidney.