Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC22A3
  • Specificity
    ab183071 detects endogenous level of total SLC22A3 protein.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Synthetic peptide within Human SLC22A3 aa 336-350. The exact sequence is proprietary. Genbank: NP_068812
    Sequence:

    PSFLDLVRTPQMRKC


    Database link: O75751

  • Positive control
    • Human liver cancer tissue lysate; Human lung cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab183071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 61 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
  • Tissue specificity
    Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Organic cation transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EMT antibody
    • EMT organic cation transporter 3 antibody
    • EMTH antibody
    • Extraneuronal monoamine transporter antibody
    • OCT 3 antibody
    • OCT3 antibody
    • Orct 3 antibody
    • Orct3 antibody
    • Organic cation transporter 3 antibody
    • S22A3_HUMAN antibody
    • SLC22 A3 antibody
    • SLC22A 3 antibody
    • Slc22a3 antibody
    • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
    • Solute carrier family 22 (organic cation transporter) member 3 antibody
    • Solute carrier family 22 member 3 antibody
    see all

Images

  • Anti-SLC22A3 antibody (ab183071) at 1/600 dilution + Human liver cancer tissue lysate at 40 µg

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 61 kDa


    Exposure time : 1 minute
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling SLC22A3 with ab183071 at a 1/40 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)

References

This product has been referenced in:
  • Sekhar GN  et al. Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB). PLoS One 12:e0173474 (2017). Read more (PubMed: 28362799) »
  • Li X  et al. Reversing the reduced level of endometrial GLUT4 expression in polycystic ovary syndrome: a mechanistic study of metformin action. Am J Transl Res 7:574-86 (2015). Read more (PubMed: 26045896) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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