Overview

  • Product nameAnti-SLC22A3 antibody
    See all SLC22A3 primary antibodies
  • Description
    Rabbit polyclonal to SLC22A3
  • Specificityab74152 detects endogenous levels of total SLC22A3 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SLC22A3 aa 275-324.

  • Positive control
    • COLO, HUVEC and HT-29 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab74152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA 1/10000.

Target

  • FunctionMediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
  • Tissue specificityExpressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EMT antibody
    • EMT organic cation transporter 3 antibody
    • EMTH antibody
    • Extraneuronal monoamine transporter antibody
    • OCT 3 antibody
    • OCT3 antibody
    • Orct 3 antibody
    • Orct3 antibody
    • Organic cation transporter 3 antibody
    • S22A3_HUMAN antibody
    • SLC22 A3 antibody
    • SLC22A 3 antibody
    • Slc22a3 antibody
    • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
    • Solute carrier family 22 (organic cation transporter) member 3 antibody
    • Solute carrier family 22 member 3 antibody
    see all

Anti-SLC22A3 antibody images

  • All lanes : Anti-SLC22A3 antibody (ab74152) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : HUVEC cell extracts
    Lane 3 : HT-29 cell extracts
    Lane 4 : HUVEC cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 30 kDa,40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SLC22A3 antibody (ab74152)

ab74152 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8% gel)
Sample Mouse Tissue lysate - whole (mouse hippocampus & cerebellum brain homogenates,)
Specification mouse hippocampus & cerebellum brain homogenates,
Treatment Samples lysed in RIPA buffer containing protease inhibitors
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Rheaclare Fraser

Verified customer

Submitted Jan 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"