Overview

  • Product nameAnti-SLC22A3 antibody [EPR6630]
    See all SLC22A3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6630] to SLC22A3
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
    • Tissue specificityExpressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
    • Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • EMT antibody
      • EMT organic cation transporter 3 antibody
      • EMTH antibody
      • Extraneuronal monoamine transporter antibody
      • OCT 3 antibody
      • OCT3 antibody
      • Orct 3 antibody
      • Orct3 antibody
      • Organic cation transporter 3 antibody
      • S22A3_HUMAN antibody
      • SLC22 A3 antibody
      • SLC22A 3 antibody
      • Slc22a3 antibody
      • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
      • Solute carrier family 22 (organic cation transporter) member 3 antibody
      • Solute carrier family 22 member 3 antibody
      see all

    Anti-SLC22A3 antibody [EPR6630] images

    • Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-SLC22A3 antibody [EPR6630] (ab124826) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human muscle lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • ab124826, at 1/100 dilution, staining SLC22A3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-SLC22A3 antibody [EPR6630] (ab124826)

    This product has been referenced in:
    • Gai Z  et al. Organic Cation Transporter 2 Overexpression May Confer an Increased Risk of Gentamicin-Induced Nephrotoxicity. Antimicrob Agents Chemother 60:5573-80 (2016). Read more (PubMed: 27401566) »
    • Aschauer L  et al. Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1. Toxicol In Vitro N/A:N/A (2014). Read more (PubMed: 25500123) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us.
    For WB 1-3%BSA in TBST and for IHC 1-3%BSA in PBST would be good for diluting the antibody.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Thank you for contacting us.
    The dilutions should always be optimized by end user. We can recommend a starting dilution of 1/500 for ab135721 and 1/50,000 for ab124826.
    I hope this information is helpful to you. Please do not hesitate to cont...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"