Anti-SLC22A3 antibody [mAbcam75402] (ab75402)

Overview

  • Product nameAnti-SLC22A3 antibody [mAbcam75402]
    See all SLC22A3 primary antibodies
  • Description
    Mouse monoclonal [mAbcam75402] to SLC22A3
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human SLC22A3.

    (Peptide available as ab107919.)

  • Positive control
    • This antibody gave a positive signal in Human Prostate Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • FunctionMediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain.
  • Tissue specificityExpressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Organic cation transporter family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EMT antibody
    • EMT organic cation transporter 3 antibody
    • EMTH antibody
    • Extraneuronal monoamine transporter antibody
    • OCT 3 antibody
    • OCT3 antibody
    • Orct 3 antibody
    • Orct3 antibody
    • Organic cation transporter 3 antibody
    • S22A3_HUMAN antibody
    • SLC22 A3 antibody
    • SLC22A 3 antibody
    • Slc22a3 antibody
    • Solute carrier family 22 (extraneuronal monoamine transporter) member 3 antibody
    • Solute carrier family 22 (organic cation transporter) member 3 antibody
    • Solute carrier family 22 member 3 antibody
    see all

Anti-SLC22A3 antibody [mAbcam75402] images

  • Anti-SLC22A3 antibody [mAbcam75402] (ab75402) at 5 µg/ml + Human prostate tissue lysate (cirrhosis) - total protein (ab30307) at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa


    Exposure time : 16 minutes
  • Overlay histogram showing JEG3 cells stained with ab75402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75402, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-SLC22A3 antibody [mAbcam75402] (ab75402)

ab75402 has not yet been referenced specifically in any publications.

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