Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC22A7
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide: LPIFLAAVPA HRCALPGAPA NFSHQDVWLE AHLPREPDGT LSSCLRFAYP, corresponding to amino acids 37-86 of Human SLC22A7

  • Positive control
    • Jurkat cell membrane lysate; Human muscle tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Mediates sodium-independent multispecific organic anion transport. Transport of prostaglandin E2, prostaglandin F2, tetracycline, bumetanide, estrone sulfate, glutarate, dehydroepiandrosterone sulfate, allopurinol, 5-fluorouracil, paclitaxel, L-ascorbic acid, salicylate, ethotrexate, and alpha-ketoglutarate.
  • Tissue specificity
    Expressed in liver and kidney.
  • Sequence similarities
    Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
  • Cellular localization
    Basolateral cell membrane. Apical side of the renal tubule.
  • Information by UniProt
  • Database links
  • Alternative names
    • hOAT2 antibody
    • liver specific transporter antibody
    • NLT antibody
    • Novel liver transporter antibody
    • OAT2 antibody
    • Organic anion transporter 2 antibody
    • Organic anion transporter member 7 antibody
    • Organic anion transporter member 7 Fragment antibody
    • S22A7_HUMAN antibody
    • SLC22A7 antibody
    • solute carrier family 22 (organic anion transporter) member 7 antibody
    • Solute carrier family 22 antibody
    • Solute carrier family 22 member 7 antibody
    see all

Images

  • All lanes : Anti-SLC22A7 antibody (ab58683) at 5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/5000 dilution

    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SLC22A7 with ab58683 at 8µg/ml. Arrows indicate positive labelled skeletal muscle cells. Magnification: X400.

  • All lanes : Anti-SLC22A7 antibody (ab58683) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

References

ab58683 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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