Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC22A8
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 493-542 (PETLNQPLPE TIEDLENWSL RAKKPKQEPE VEKASQRIPL QPHGPGLGSS) of human SLC22A8 (NP_004245).

  • Positive control
    • Fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • Function
    Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).
  • Tissue specificity
    Expressed in kidney.
  • Sequence similarities
    Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
  • Cellular localization
    Basolateral cell membrane. Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • hOAT3 antibody
    • OAT3 antibody
    • Organic anion transporter 3 antibody
    • S22A8_HUMAN antibody
    • SLC22A8 antibody
    • Solute carrier family 22 (organic anion transporter), member 8 antibody
    • Solute carrier family 22 member 8 antibody
    see all

Images

  • Anti-SLC22A8 antibody (ab83789) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Xu D  et al. An Essential Role of Nedd4-2 in the Ubiquitination, Expression, and Function of Organic Anion Transporter-3. Mol Pharm 13:621-30 (2016). Read more (PubMed: 26651153) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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