Overview

  • Product nameAnti-SLC23A2 antibody
  • Description
    Mouse polyclonal to SLC23A2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    WKGLRKSDNSRSSDEDSQAT

    , corresponding to amino acids 630-650 of Human SLC23A2

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 70 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceSodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na(+) for each ascorbate.
  • Cellular localizationMembrane; multi-pass membrane protein.
  • Database links
  • Alternative names
    • KIAA0238 antibody
    • Na(+)/L ascorbic acid transporter 2 antibody
    • NBTL1 antibody
    • RP1 237C24.1 antibody
    • Sodium dependent vitamin C transporter 2 antibody
    • Sodium dependent vitamin C transporter 2 variant II antibody
    • Solute carrier family 23 (nucleobase transporters) member 2 antibody
    • SVCT2 antibody
    • Yolk sac permease like molecule 2 antibody
    • YSPL2 antibody
    see all

Anti-SLC23A2 antibody images

  • All lanes : Anti-SLC23A2 antibody (ab43748) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 500ng of a recombinant His tag fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant His tag fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

    Predicted band size : 70 kDa

References for Anti-SLC23A2 antibody (ab43748)

ab43748 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"