The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCalcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle.
Tissue specificityHigh levels in heart and skeletal muscle, low in brain and very low in kidney.
Involvement in diseaseDefects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system.
Sequence similaritiesBelongs to the mitochondrial carrier family. Contains 4 EF-hand domains. Contains 3 Solcar repeats.
Solute carrier family 25 (aspartate/glutamate carrier) member 12 antibody
Solute carrier family 25 (mitochondrial carrier Aralar) member 12 antibody
Solute carrier family 25 member 12 antibody
solute carrier family 25, member 12 antibody
Anti-SLC25A12 antibody images
Western blot - SLC25A12 antibody (ab90095)
Anti-SLC25A12 antibody (ab90095) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 75 kDa
References for Anti-SLC25A12 antibody (ab90095)
This product has been referenced in:
Povlsen JA et al. Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation. PLoS One8:e64093 (2013).
Read more (PubMed: 23704975) »
Zhong Q & Kowluru RA Diabetic retinopathy and damage to mitochondrial structure and transport machinery. Invest Ophthalmol Vis Sci52:8739-46 (2011).
Read more (PubMed: 22003103) »