Overview

  • Product nameAnti-SLC25A22 antibody
    See all SLC25A22 primary antibodies
  • Description
    Rabbit polyclonal to SLC25A22
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: AGCGAGTCQV IVTTPMEMLK IQLQDAGRIA AQRKILAAQG QLSAQGGAQP, corresponding to N terminal amino acids 109-158 of Human SLC25A22

  • Positive control
    • Jurkat cell lysate; Human muscle tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab60332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • FunctionInvolved in the transport of glutamate across the inner mitochondrial membrane. Glutamate is cotransported with H(+).
  • Tissue specificityHighly expressed in most tissues.
  • Involvement in diseaseDefects in SLC25A22 are the cause of epileptic encephalopathy early infantile type 3 (EIEE3) [MIM:609304]; also known as early myoclonic encephalopathy (EME) or neonatal epilepsy with suppression-burst pattern. Severe neonatal epilepsies with suppression-burst pattern are early-onset epileptic syndromes characterized by a typical EEG pattern. The suppression-burst pattern of the EEG is characterized by higher-voltage bursts of slow waves mixed with multifocal spikes alternating with isoelectric suppression phases. EME is characterized by a very early onset, erratic and fragmentary myoclonus, massive myoclonus, partial motor seizures and late tonic spasms. The prognosis of EME is poor, with no effective treatment, and children with the condition either die within 1 to 2 years after birth or survive in a persistent vegetative state. EME inheritance is autosomal recessive.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GC 1 antibody
    • GC-1 antibody
    • GC1 antibody
    • GHC1_HUMAN antibody
    • Glutamate/H(+) symporter 1 antibody
    • Mitochondrial glutamate carrier 1 antibody
    • SLC25A22 antibody
    • Solute carrier family 25 member 22 antibody
    see all

Anti-SLC25A22 antibody images

  • All lanes : Anti-SLC25A22 antibody (ab60332) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SLC25A22 with ab60332 at 4-8µg/ml. Arrows indicate positively stained skeletal muscle cells. Magnification: 400X.

References for Anti-SLC25A22 antibody (ab60332)

ab60332 has not yet been referenced specifically in any publications.

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