Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC25A28
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: LLNTQESLAL NSHITGHITG MASAFRTVYQ VGGVTAYFRG VQARVIYQIP, corresponding to C terminal amino acids 289-338 of Human SLC25A28 (NP_112489)

  • Positive control
    • WB: MCF7 cell lyaste. IHC-P: Human brain cortex and human kidney tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab80467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells.
  • Cellular localization
    Mitochondrion inner membrane; Multi pass membrane protein. Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
  • Database links
  • Alternative names
    • MFRN2 antibody
    • Mitochondrial iron transporter 2 antibody
    • Mitochondrial RNA splicing protein 3/4 antibody
    • Mitoferrin 2 antibody
    • MRS3/4 antibody
    • MRS4L antibody
    • NPD016 antibody
    • Putative mitochondrial solute carrier antibody
    see all

Images

  • Anti-SLC25A28 antibody (ab80467) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab80467 at 5 µg/ml staining SLC25A28 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ab80467 at 5 µg/ml staining SLC25A28 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

References

ab80467 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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