Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC25A28
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 216-265 (WQNEGAGAFY RSYTTQLTMN VPFQAIHFMT YEFLQEHFNP QRRYNPSSHV) of Human SLC25A28 (NP_112489).

  • Positive control
    • Human fetal stomach lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    SLC25A28 is a mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells.
  • Cellular localization
    Mitochondrion inner membrane; Multi pass membrane protein. Note: Isoform 1 and isoform 2 are both localized in the mitochondrion.
  • Database links
  • Alternative names
    • MFRN2 antibody
    • Mitochondrial iron transporter 2 antibody
    • Mitochondrial RNA splicing protein 3/4 antibody
    • Mitoferrin 2 antibody
    • MRS3/4 antibody
    • MRS4L antibody
    • NPD016 antibody
    • Putative mitochondrial solute carrier antibody
    see all

Images

  • Anti-SLC25A28 antibody (ab90170) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa

References

ab90170 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

melissa kelly

Verified customer

Submitted Aug 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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