The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
Use a concentration of 0.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
FunctionMitochondrial carrier required during erythropoiesis. Probably involved in the biosynthesis of heme, possibly by facilitating 5-aminolevulinate (ALA) production. May act by importing glycine into mitochondria or by exchanging glycine for ALA across the mitochondrial inner membrane.
Tissue specificityPreferentially expressed in erythroid cells.
Involvement in diseaseAnemia, sideroblastic, pyridoxine-refractory, autosomal recessive
Sequence similaritiesBelongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily. Contains 3 Solcar repeats.