Overview

  • Product name
    Anti-SLC25A45 antibody
  • Description
    Rabbit polyclonal to SLC25A45
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cat, Dog
  • Immunogen

    A region within synthetic peptide: QGMLDCMVSS IRQEGLGVFF RGVTINSARA FPVNAVTFLS YEYLLRWWG, corresponding to C terminal amino acids 198-246 of Human SLC25A45

  • Positive control
    • HepG2 cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab60135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately <36 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    Belongs to the mitochondrial carrier family, its but function remains unknown. There are four named isoforms.
  • Cellular localization
    Mitochondrion inner membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • LOC283130 antibody
    • SLC25A45 antibody
    • Solute carrier family 25 member 45 antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC25A45 with ab60135 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • Anti-SLC25A45 antibody (ab60135) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : <36 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%

References

ab60135 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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