Overview

  • Product nameAnti-SLC26A3 antibody
    See all SLC26A3 primary antibodies
  • Description
    Mouse monoclonal to SLC26A3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: TQFPKCSTLA NIGRTNIYKN KKDYYDMYEP EGVKIFRCPS PIYFANIGFF RRKLIDAVGF SPLRILRKRN KALRKIRKLQ KQGLLQVTPK GFICTVDT, corresponding to amino acids 503-601 of Human SLC26A3

Properties

Applications

Our Abpromise guarantee covers the use of ab54836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 1 - 2 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

  • Target

    • FunctionChloride/bicarbonate exchanger. Involved in absorbtion of in the colon. Helps mediate electrolyte and fluid absorption.
    • Involvement in diseaseDefects in SLC26A3 are the cause of diarrhea type 1 (DIAR1) [MIM:214700]; also known as congenital chloride diarrhea (CLD). DIAR1 is a disease characterized by voluminous watery stools containing an excess of chloride. The children with this disease are often premature.
    • Sequence similaritiesBelongs to the SLC26A/SulP transporter (TC 2.A.53) family.
      Contains 1 STAS domain.
    • Developmental stageExpression is significantly decreased in adenomas (polyps) and adenocarcinomas of the colon.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Chloride anion exchanger antibody
      • CLD antibody
      • Congenital chloride diarrhea antibody
      • Down regulated in adenoma antibody
      • Down regulated in adenoma protein antibody
      • Down-regulated in adenoma antibody
      • DRA antibody
      • Protein DRA antibody
      • S26A3_HUMAN antibody
      • SLC26A3 antibody
      • Solute carrier family 26 member 3 antibody
      see all

    Anti-SLC26A3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab54836 SLC26A3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • ab54836 at 1.8 ug/ml staining SLC26A3 in human spleen tissue sections by Immunohistochemistry (Formalin /PFA fixed paraffin embedded sections).

    References for Anti-SLC26A3 antibody (ab54836)

    ab54836 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Purified protein (Caco-2 (colorectal adenocarcinoma) Cells)
    Loading amount 50 µg
    Specification Caco-2 (colorectal adenocarcinoma) Cells
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Aug 03 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (intestine (caecum))
    Loading amount 50 µg
    Specification intestine (caecum)
    Gel Running Conditions Reduced Denaturing (10% Tris-Glycine)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 03 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"