Anti-SLC27A5/BAL antibody (ab74869)
Key features and details
- Rabbit polyclonal to SLC27A5/BAL
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC27A5/BAL antibody
See all SLC27A5/BAL primary antibodies -
Description
Rabbit polyclonal to SLC27A5/BAL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: human liver carcinoma tissue. WB: extracts from HepG2 cells. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 75 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 75 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. -
Tissue specificity
Predominantly expressed in liver. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 10998 Human
- Omim: 603314 Human
- SwissProt: Q9Y2P5 Human
- Unigene: 292177 Human
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Alternative names
- ACSB antibody
- ACSVL6 antibody
- BA CoA ligase antibody
see all
Images
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All lanes : Anti-SLC27A5/BAL antibody (ab74869) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 75 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Immunohistochemistry analysis of SLC27A5/BAL in paraffin-embedded human liver carcinoma tissue using ab74869, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescence analysis of SLC27A5/BAL in A549 cells, using ab74869, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab74869 has been referenced in 2 publications.
- Zhang F et al. Identifying SLC27A5 as a potential prognostic marker of hepatocellular carcinoma by weighted gene co-expression network analysis and in vitro assays. Cancer Cell Int 21:174 (2021). PubMed: 33731144
- Przybylla S et al. Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches. PLoS One 11:e0159778 (2016). IP ; Human . PubMed: 27472061