• Product nameAnti-SLC27A5 antibody
    See all SLC27A5 primary antibodies
  • Description
    Rabbit polyclonal to SLC27A5
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human SLC27A5

  • Positive control
    • IHC-P: human liver carcinoma tissue. WB: extracts from HepG2 cells. ICC/IF: A549 cells.



Our Abpromise guarantee covers the use of ab74869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.


  • FunctionAcyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
  • Tissue specificityPredominantly expressed in liver.
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACSB antibody
    • ACSVL6 antibody
    • BA CoA ligase antibody
    • BA-CoA ligase antibody
    • BACS antibody
    • BAL antibody
    • Bile acid CoA ligase antibody
    • Bile acid-CoA ligase antibody
    • Bile acyl CoA synthetase antibody
    • Bile acyl-CoA synthetase antibody
    • Cholate CoA ligase antibody
    • Cholate--CoA ligase antibody
    • FACVL3 antibody
    • FATP 5 antibody
    • FATP-5 antibody
    • FATP5 antibody
    • Fatty acid Coenzyme A ligase very long chain 3 antibody
    • Fatty acid transport protein 5 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • FLJ22987 antibody
    • S27A5_HUMAN antibody
    • Slc27a5 antibody
    • Solute carrier family 27 (fatty acid transporter) member 5 antibody
    • Solute carrier family 27 member 5 antibody
    • Very long chain acyl CoA synthetase homolog 2 antibody
    • Very long chain acyl CoA synthetase related protein antibody
    • very long-chain 3 antibody
    • Very long-chain acyl-CoA synthetase homolog 2 antibody
    • Very long-chain acyl-CoA synthetase-related protein antibody
    • VLACS related antibody
    • VLACS-related antibody
    • VLACSR antibody
    • VLCS H2 antibody
    • VLCS-H2 antibody
    • VLCSH2 antibody
    see all

Anti-SLC27A5 antibody images

  • Immunohistochemistry analysis of SLC27A5 in paraffin-embedded human liver carcinoma tissue using ab74869, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-SLC27A5 antibody (ab74869) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 75 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of SLC27A5 in A549 cells, using ab74869, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-SLC27A5 antibody (ab74869)

ab74869 has not yet been referenced specifically in any publications.

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Thank you very much for your call yesterday and for your interest in ab74869.

I've heard back from the lab, and unfortunately the immunogen of this antibody only shares 57% identity with the mouse sequence, so it's not highly likely to cross-...

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