Overview

  • Product nameAnti-SLC27A5 antibody
    See all SLC27A5 primary antibodies
  • Description
    Rabbit polyclonal to SLC27A5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 611-660 (KLYQHVRAWL PAYATPHFIR IQDAMEVTST FKLMKTRLVR EGFNVGIVVD) of Human SLC27A5 (NP_036386)

  • Positive control
    • OVCAR3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab97884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAcyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
  • Tissue specificityPredominantly expressed in liver.
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACSB antibody
    • ACSVL6 antibody
    • BA CoA ligase antibody
    • BA-CoA ligase antibody
    • BACS antibody
    • BAL antibody
    • Bile acid CoA ligase antibody
    • Bile acid-CoA ligase antibody
    • Bile acyl CoA synthetase antibody
    • Bile acyl-CoA synthetase antibody
    • Cholate CoA ligase antibody
    • Cholate--CoA ligase antibody
    • FACVL3 antibody
    • FATP 5 antibody
    • FATP-5 antibody
    • FATP5 antibody
    • Fatty acid Coenzyme A ligase very long chain 3 antibody
    • Fatty acid transport protein 5 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • FLJ22987 antibody
    • S27A5_HUMAN antibody
    • Slc27a5 antibody
    • Solute carrier family 27 (fatty acid transporter) member 5 antibody
    • Solute carrier family 27 member 5 antibody
    • Very long chain acyl CoA synthetase homolog 2 antibody
    • Very long chain acyl CoA synthetase related protein antibody
    • very long-chain 3 antibody
    • Very long-chain acyl-CoA synthetase homolog 2 antibody
    • Very long-chain acyl-CoA synthetase-related protein antibody
    • VLACS related antibody
    • VLACS-related antibody
    • VLACSR antibody
    • VLCS H2 antibody
    • VLCS-H2 antibody
    • VLCSH2 antibody
    see all

Anti-SLC27A5 antibody images

  • Anti-SLC27A5 antibody (ab97884) at 1 µg/ml + OVCAR3 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa

References for Anti-SLC27A5 antibody (ab97884)

This product has been referenced in:
  • Aleksunes LM  et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »

See 1 Publication for this product

Product Wall

Thank you very much for your recent review of ab97884. I am sorry to see that the results were not satisfactory.

I would like to offer a couple of suggestions that might improve the results, though you may have already tried optimizing the pr...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Xia Wen

Verified customer

Submitted Jul 02 2012

Thank you for your reply and for submitting your Abreviews. The discount codes I had sent you are now active and can each be used to receive a free antibody on a future order. If you are interested in testing any other antibodies, please let me know an...

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For ab83882: DISCOUNT CODE: ***
For ab97884: DISCOUNT CODE: ***
Expiration date: October 5th, 2012

I am very pleased to hear you would like to accept our offer and test ab83882 and ab97884 in mouse.Each code will give you: 1 free pri...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"