Overview

  • Product name
    Anti-SLC27A5 antibody [CL0213]
    See all SLC27A5 primary antibodies
  • Description
    Mouse monoclonal [CL0213] to SLC27A5
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 223-345 of Human SLC27A5 (UniProt ID: Q9Y2P5).

  • Positive control
    • Human liver tissue; Human liver tissue lysate.
  • General notes
    The antibody solution should be gently mixed before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab154140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/2500. Predicted molecular weight: 75 kDa.

Target

  • Function
    Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.
  • Tissue specificity
    Predominantly expressed in liver.
  • Sequence similarities
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACSB antibody
    • ACSVL6 antibody
    • BA CoA ligase antibody
    • BA-CoA ligase antibody
    • BACS antibody
    • BAL antibody
    • Bile acid CoA ligase antibody
    • Bile acid-CoA ligase antibody
    • Bile acyl CoA synthetase antibody
    • Bile acyl-CoA synthetase antibody
    • Cholate CoA ligase antibody
    • Cholate--CoA ligase antibody
    • FACVL3 antibody
    • FATP 5 antibody
    • FATP-5 antibody
    • FATP5 antibody
    • Fatty acid Coenzyme A ligase very long chain 3 antibody
    • Fatty acid transport protein 5 antibody
    • Fatty-acid-coenzyme A ligase antibody
    • FLJ22987 antibody
    • S27A5_HUMAN antibody
    • Slc27a5 antibody
    • Solute carrier family 27 (fatty acid transporter) member 5 antibody
    • Solute carrier family 27 member 5 antibody
    • Very long chain acyl CoA synthetase homolog 2 antibody
    • Very long chain acyl CoA synthetase related protein antibody
    • very long-chain 3 antibody
    • Very long-chain acyl-CoA synthetase homolog 2 antibody
    • Very long-chain acyl-CoA synthetase-related protein antibody
    • VLACS related antibody
    • VLACS-related antibody
    • VLACSR antibody
    • VLCS H2 antibody
    • VLCS-H2 antibody
    • VLCSH2 antibody
    see all

Images

  • Immunohistochemical analysis of Human liver tissue labeling SLC27A5 with ab154140 at 1/500 dilution.
  • Immunohistochemical analysis of Human pancreas tissue showing no labeling of SLC27A5 using ab154140 at 1/500 dilution (negative control).
  • Anti-SLC27A5 antibody [CL0213] (ab154140) at 1/1000 dilution + Human liver tissue lysate

    Predicted band size: 75 kDa

References

ab154140 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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