The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
FunctionInvolved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. Thought to function as the predominant fatty acid protein transporter in heart.
Tissue specificityStrongly expressed in heart and localizes to cardiac myocytes. Expressed at moderate levels in placenta, testis, and adrenal glands. Expressed at very low levels in kidney, bladder and uterus.
Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
Cellular localizationMembrane. Cell membrane > sarcolemma. In heart is exclusively located on the sarcolemma in areas juxtaposed with small blood vessels where it colocalizes CD36.