Overview

  • Product nameAnti-SLC35C1 antibody
    See all SLC35C1 primary antibodies
  • Description
    Rabbit polyclonal to SLC35C1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: RALQIALVVS LYSVTSISMV FLNKYLLDSP SLRLDTPIFV TFYQCLVTTL, corresponding to N terminal amino acids 37-86 of Human SLC35C1

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab60336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

Target

  • RelevanceSLC35C1 is involved in GDP-fucose import from the cytoplasm into the Golgi lumen. Defects in SLC35C1 are the cause of congenital disorder of glycosylation type 2C (CDG2C), also known as leukocyte adhesion deficiency type II (LAD2). The clinical features of CDG2C include mental retardation, short stature, facial stigmata, and recurrent bacterial peripheral infections with persistently elevated peripheral leukocytes.
  • Cellular localizationGolgi apparatus membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • FLJ11320 antibody
    • FLJ14841 antibody
    • FUCT1 antibody
    • GDP fucose transporter 1 antibody
    • Solute carrier family 35 member C1 antibody
    see all

Anti-SLC35C1 antibody images

  • All lanes : Anti-SLC35C1 antibody (ab60336) at 1.25 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

References for Anti-SLC35C1 antibody (ab60336)

ab60336 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic fibroblasts)
Gel Running Conditions Reduced Denaturing (12% Tris-Glycine)
Loading amount 30 µg
Specification embryonic fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"