Anti-SLC36A1 antibody - N-terminal (ab189441)
Key features and details
- Rabbit polyclonal to SLC36A1 - N-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC36A1 antibody - N-terminal -
Description
Rabbit polyclonal to SLC36A1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLC36A1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q7Z2H8 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: NCI-460 cells. Flow Cyt (Intra): NCI-460 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/10 - 1/50.
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WB |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/10 - 1/50. |
WB
Use at an assay dependent concentration. |
Target
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Function
Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid. -
Sequence similarities
Belongs to the amino acid/polyamine transporter 2 family. -
Cellular localization
Cell membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 206358 Human
- Omim: 606561 Human
- SwissProt: Q7Z2H8 Human
- Unigene: 269004 Human
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Alternative names
- Dct1 antibody
- hPAT1 antibody
- LYAAT1 antibody
see all
Images
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney sections labeling SLC36A1 using ab189441 at 5 µg/ml.
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Immunofluorescent staining of NCI-460 cells labeling SLC36A1 using ab189441 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Intracellular flow cytometric analysis of NCI-460 cells (right histogram) compared to a negative control cell (left histogram) labeling SLC36A1 using ab189441 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab189441 has not yet been referenced specifically in any publications.