The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 53 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/10 - 1/50.
Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid.
Belongs to the amino acid/polyamine transporter 2 family.
Immunofluorescent staining of NCI-460 cells labeling SLC36A1 using ab189441 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-460 cells (right histogram) compared to a negative control cell (left histogram) labeling SLC36A1 using ab189441 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.