Anti-SLC36A1 antibody - N-terminal (ab189441)

Overview

  • Product name
    Anti-SLC36A1 antibody - N-terminal
    See all SLC36A1 primary antibodies
  • Description
    Rabbit polyclonal to SLC36A1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC36A1 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    MSTQRLRNED YHDYSSTDVS PEESPSEGLN


    Database link: Q7Z2H8

  • Positive control
    • Human kidney tissue. NCI-460 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function
    Neutral amino acid/proton symporter. Has a pH-dependent electrogenic transport activity for small amino acids such as glycine, alanine and proline. Besides small apolar L-amino acids, it also recognize their D-enantiomers and selected amino acid derivatives such as gamma-aminobutyric acid.
  • Sequence similarities
    Belongs to the amino acid/polyamine transporter 2 family.
  • Cellular localization
    Cell membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dct1 antibody
    • hPAT1 antibody
    • LYAAT1 antibody
    • PAT1 antibody
    • Proton-coupled amino acid transporter 1 antibody
    • Proton/amino acid transporter 1 antibody
    • S36A1_HUMAN antibody
    • Slc36a1 antibody
    • Solute carrier family 36 member 1 antibody
    • TRAMD3 antibody
    see all

Images

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human kidney sections labeling SLC36A1 using ab189441 at 5 µg/ml.

  • Immunofluorescent staining of NCI-460 cells labeling SLC36A1 using ab189441 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • Flow cytometric analysis of NCI-460 cells (right histogram) compared to a negative control cell (left histogram) labeling SLC36A1 using ab189441 at 1/10 dilution. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

     

References

ab189441 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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