Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC38A1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: TAIFGYLTFY DNVQSDLLHK YQSKDDILIL TVRLAVIVAV ILTVPVLFFT, corresponding to internal sequence amino acids 325-374 of Human SLC38A1

  • Positive control
    • HepG2 cells. Kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA.
  • Tissue specificity
    Expressed in the cerebral cortex by pyramidal and GABAergic neurons, astrocytes and other non-neuronal cells (at protein level). Expressed in placenta, heart, lung, skeletal muscle, spleen, stomach and testis.
  • Sequence similarities
    Belongs to the amino acid/polyamine transporter 2 family.
  • Cellular localization
    Cell membrane. Restricted to the somatodendritic compartment of neurons. Found in the cellular processes of neurons in the developing brain (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • Amino acid transporter A1 antibody
    • Amino acid transporter system A1 antibody
    • ATA 1 antibody
    • ATA1 antibody
    • N-system amino acid transporter 2 antibody
    • NAT 2 antibody
    • NAT2 antibody
    • S38A1_HUMAN antibody
    • SAT 1 antibody
    • SAT1 antibody
    • SLC38A1 antibody
    • SNAT 1 antibody
    • SNAT1 antibody
    • Sodium-coupled neutral amino acid transporter 1 antibody
    • Solute carrier family 38 member 1 antibody
    • System A amino acid transporter 1 antibody
    • System N amino acid transporter 1 antibody
    see all

Images

  • Anti-SLC38A1 antibody (ab59721) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A1 with ab59721 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

References

This product has been referenced in:
  • Chen Y  et al. The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma. Oncotarget 6:20419-33 (2015). IHC . Read more (PubMed: 25971332) »
  • Zhou J  et al. Overexpression of Prdx1 in hilar cholangiocarcinoma: a predictor for recurrence and prognosis. Int J Clin Exp Pathol 8:9863-74 (2015). Read more (PubMed: 26617696) »

See all 4 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (placenta, day 20)
Loading amount
30 µg
Specification
placenta, day 20
Gel Running Conditions
Reduced Denaturing (5%)
Blocking step
PVA as blocking agent for 1 minute(s) · Concentration: 1µg/mL
Username

Dr. Heidi Engelhardt

Verified customer

Submitted Feb 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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