Overview

  • Product nameAnti-SLC38A4 antibody
  • Description
    Rabbit polyclonal to SLC38A4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: RRKMQTVSNI SITGMLVMYL LAALFGYLTF YGEVEDELLH AYSKVYTLDI, corresponding to C terminal amino acids 361-410 of Human SLC38A4

  • Positive control
    • Jurkat cell lysate; human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab58785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceSLC38A4 is found predominantly in liver and transports both cationic and neutral amino acids. The transport of cationic amino acids by SLC38A4 is Na+ and pH independent, while the transport of neutral amino acids is Na+ and pH dependent. It has a broad specificity, with a preference for Ala, followed by His, Cys, Asn, Ser, Gly, Val, Thr, Gln and Met.
  • Cellular localizationCell Membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • Amino acid transporter A3 antibody
    • Na(+)-coupled neutral amino acid transporter 4 antibody
    • Sodium-coupled neutral amino acid transporter 4 antibody
    • Solute carrier family 38 member 4 antibody
    • System N amino acid transporter 3 antibody
    see all

Anti-SLC38A4 antibody images

  • Lane 1 : marker
    Lane 2 : Anti-SLC38A4 antibody (ab58785) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 61 kDa
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC38A4 with ab58785 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

References for Anti-SLC38A4 antibody (ab58785)

This product has been referenced in:
  • Desforges M  et al. The SNAT4 isoform of the system A amino acid transporter is functional in human placental microvillous plasma membrane. J Physiol 587:61-72 (2009). WB, IHC-P ; Human . Read more (PubMed: 19015196) »

See 1 Publication for this product

Product Wall



Unfortunately, at this time, the only data our lab has is the western blot and IHC data with human samples. We have not conducted additional experiments to address why the staining seems to be mainly cytoplasmic. Additionally, from the 14aa p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"