The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays an important role in cellular zinc homeostasis as a zinc transporter. Regulated in response to zinc availability.
Tissue specificityHighly expressed in kidney, small intestine, stomach, colon, jejunum and duodenum.
Involvement in diseaseAcrodermatitis enteropathica, zinc-deficiency type
Sequence similaritiesBelongs to the ZIP transporter (TC 2.A.5) family.
Cellular localizationCell membrane. Recycling endosome membrane. Colocalized with TFRC in the recycling endosomes. Cycles between endosomal compartments and the plasma membrane in response to zinc availability.