Our Abpromise guarantee covers the use of ab58695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 62-69 kDa).

 Incubate for 2hrs 

ELISA Use at an assay dependent concentration.


  • Function
    May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin).
  • Tissue specificity
    Expressed in placenta, intestine, muscle and spleen.
  • Involvement in disease
    Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4) [MIM:606069]. HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation.
  • Sequence similarities
    Belongs to the SLC40A transporter family.
  • Cellular localization
    Cell membrane. Localized to the basolateral membrane of polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ferroportin 1 antibody
    • Ferroportin antibody
    • Ferroportin-1 antibody
    • FPN1 antibody
    • HFE4 antibody
    • IREG1 antibody
    • iron regulated gene 1 antibody
    • Iron regulated transporter 1 antibody
    • Iron-regulated transporter 1 antibody
    • MST079 antibody
    • MSTP079 antibody
    • MTP1 antibody
    • putative ferroportin 1 variant IIIA antibody
    • putative ferroportin 1 variant IIIB antibody
    • S40A1_HUMAN antibody
    • SLC11A3 antibody
    • SLC11A3, formerly antibody
    • SLC40A1 antibody
    • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3 antibody
    • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3, formerly antibody
    • solute carrier family 40 (iron-regulated transporter), member 1 antibody
    • Solute carrier family 40 member 1 antibody
    see all

Anti-SLC40A1 antibody images

  • Anti-SLC40A1 antibody (ab58695) at 1 µg/ml + Human colon tissue lysate - total protein (ab30051) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 62-69 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)

References for Anti-SLC40A1 antibody (ab58695)

This product has been referenced in:
  • Scheers N & Sandberg AS Iron transport through ferroportin is induced by intracellular ascorbate and involves IRP2 and HIF2a. Nutrients 6:249-60 (2014). ELISA ; Human . Read more (PubMed: 24394537) »
  • Darshan D  et al. Reduced expression of ferroportin-1 mediates hyporesponsiveness of suckling rats to stimuli that reduce iron absorption. Gastroenterology 141:300-9 (2011). WB ; Rat . Read more (PubMed: 21570398) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

I am sorry that you are having issues with these antibodies in western blot. Having looked over all of the protocol information you sent previously and I cannot think of anything else that may prove useful. I was jus...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009


Sign up