Overview

  • Product name
  • Description
    Rabbit polyclonal to SLC40A1
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human SLC40A1 (N terminal).

  • Positive control
    • Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin).
  • Tissue specificity
    Expressed in placenta, intestine, muscle and spleen.
  • Involvement in disease
    Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4) [MIM:606069]. HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation.
  • Sequence similarities
    Belongs to the SLC40A transporter family.
  • Cellular localization
    Cell membrane. Localized to the basolateral membrane of polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ferroportin 1 antibody
    • Ferroportin antibody
    • Ferroportin-1 antibody
    • FPN1 antibody
    • HFE4 antibody
    • IREG1 antibody
    • iron regulated gene 1 antibody
    • Iron regulated transporter 1 antibody
    • Iron-regulated transporter 1 antibody
    • MST079 antibody
    • MSTP079 antibody
    • MTP1 antibody
    • putative ferroportin 1 variant IIIA antibody
    • putative ferroportin 1 variant IIIB antibody
    • S40A1_HUMAN antibody
    • SLC11A3 antibody
    • SLC11A3, formerly antibody
    • SLC40A1 antibody
    • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3 antibody
    • solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3, formerly antibody
    • solute carrier family 40 (iron-regulated transporter), member 1 antibody
    • Solute carrier family 40 member 1 antibody
    see all

Anti-SLC40A1 antibody images

  • ab78066, at 20µg/ml, staining SLC40A1 in human skeletal muscle by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
    A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ICC/IF image of ab78066 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78066, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical staining of paraffin embedded, formalin fixed, kidney renal tubular epithelium using ab78066 at 20 µg/mL.

  • Negative control - immunohistochemical staining of paraffin embedded, formalin fixed, breast epithelium using ab78066 at 20 µg/mL.

References for Anti-SLC40A1 antibody (ab78066)

This product has been referenced in:
  • McCarthy RC & Kosman DJ Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux. J Biol Chem 288:17932-40 (2013). WB ; Human . Read more (PubMed: 23640881) »
  • Guo W  et al. Testosterone administration inhibits hepcidin transcription and is associated with increased iron incorporation into red blood cells. Aging Cell 12:280-91 (2013). WB . Read more (PubMed: 23399021) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Human Tissue sections (Liver)
Specification
Liver
Permeabilization
No
Fixative
10% NBF
Username

Abcam user community

Verified customer

Submitted Sep 19 2014

Thank you for contacting Abcam regarding ab78066.


I am sorry that you have been experiencing difficulties with this antibody in IHC of frozen sections. Unfortunately, this antibody has not been tested in IHC of frozen sections and it is t...

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