Anti-SLC40A1 antibody (ab78066)
Key features and details
- Rabbit polyclonal to SLC40A1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC40A1 antibody
See all SLC40A1 primary antibodies -
Description
Rabbit polyclonal to SLC40A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human SLC40A1 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). -
Tissue specificity
Expressed in placenta, intestine, muscle and spleen. -
Involvement in disease
Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4) [MIM:606069]. HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. -
Sequence similarities
Belongs to the SLC40A transporter family. -
Cellular localization
Cell membrane. Localized to the basolateral membrane of polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 30061 Human
- Entrez Gene: 170840 Rat
- Omim: 604653 Human
- SwissProt: Q9NP59 Human
- SwissProt: Q923U9 Rat
- Unigene: 643005 Human
- Unigene: 15324 Rat
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Alternative names
- Ferroportin 1 antibody
- Ferroportin antibody
- Ferroportin-1 antibody
see all
Images
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ab78066, at 20µg/ml, staining SLC40A1 in human skeletal muscle by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
A heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. -
Immunohistochemical staining of paraffin embedded, formalin fixed, kidney renal tubular epithelium using ab78066 at 20 µg/mL.
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Negative control - immunohistochemical staining of paraffin embedded, formalin fixed, breast epithelium using ab78066 at 20 µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab78066 has been referenced in 23 publications.
- Zhang G et al. 1-2 T static magnetic field combined with Ferumoxytol prevent unloading-induced bone loss by regulating iron metabolism in osteoclastogenesis. J Orthop Translat 38:126-140 (2023). PubMed: 36381248
- Zheng J et al. Norcantharidin down-regulates iron contents in the liver and spleen of lipopolysaccharide-treated mice. Redox Rep 27:119-127 (2022). PubMed: 35735222
- Pei Z et al. Inhibition of ferroptosis and iron accumulation alleviates pulmonary fibrosis in a bleomycin model. Redox Biol 57:102509 (2022). PubMed: 36302319
- Li D et al. Nuciferine protects against folic acid-induced acute kidney injury by inhibiting ferroptosis. Br J Pharmacol 178:1182-1199 (2021). PubMed: 33450067
- Wu A et al. Fibroblast growth factor 21 attenuates iron overload-induced liver injury and fibrosis by inhibiting ferroptosis. Redox Biol 46:102131 (2021). PubMed: 34530349