Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human SLC40A1.
(Peptide available as ab97416.)
Our Abpromise guarantee covers the use of ab85370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 63 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24036496
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab85370 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.