Overview

  • Product name
    Anti-SLC41A1 antibody
  • Description
    Rabbit polyclonal to SLC41A1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A synthetic peptide corresponding to a region within the N-terminal sequence 35-84 (TSEFLGPDGAGVEVVIESRANAKGVREEDALLENGSQSNESDDVSTDRG P) of Human SLC41A1 (NP_776253).

  • Positive control
    • 721_B Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

Anti-SLC41A1 antibody images

  • Anti-SLC41A1 antibody (ab83701) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + 721_B Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SLC41A1 antibody (ab83701)

ab83701 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Whole embryo 6 days post fertilization)
Gel Running Conditions
Non-reduced Denaturing (10 % SDS PAGE)
Loading amount
6000 µg
Specification
Whole embryo 6 days post fertilization
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Greg Bonde

Verified customer

Submitted Aug 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up