Overview

  • Product name
    Anti-SLC43A3 antibody
  • Description
    Rabbit polyclonal to SLC43A3
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Dog
  • Immunogen

    A region within synthetic peptide: MAGQGLPLHV ATLLTGLLEC LGFAGVLFGW PSLVFVFKNE DYFKDLCGPD, corresponding to N terminal amino acids 1-50 of Human SLC43A3

  • Positive control
    • Jurkat cells; Lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    SLC43A3 is a putative transporter, belonging to the SLC43A transporter family. It is highly expressed in macrophages.
  • Cellular localization
    Multi-pass membrane protein
  • Database links
  • Alternative names
    • DKFZp762A227 antibody
    • EEG1 antibody
    • FOAP 13 antibody
    • likely ortholog of mouse embryonic epithelial gene 1 antibody
    • PRO1659 antibody
    • SEEEG 1 antibody
    • solute carrier family 43 member 3 antibody
    see all

Images

  • All lanes : Anti-SLC43A3 antibody (ab60302) at 2.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling SLC43A3 with ab60302 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

References

ab60302 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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