Overview

  • Product name
    Anti-SLC46A3 antibody
  • Description
    Rabbit polyclonal to SLC46A3
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human SLC46A3 (MKILFVEPAI FLSAFAMTLT GPLTTQYVYR RIWEETGNYT FSSDSNISEC)

  • Positive control
    • Jurkat cell lysate. Human kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab62301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Relevance
    SLC46A3 (Solute carrier family 46 member 3) is a member of the major facilitator superfamily and SLC46A family. Its function remains unknown.
  • Cellular localization
    Membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • FKSG 16 antibody
    • FKSG16 antibody
    • FLJ42613 antibody
    • OTTHUMP00000018184 antibody
    • RP11 97E23.2 antibody
    • Solute carrier family 46 member 3 antibody
    see all

Images

  • All lanes : SLC46A3 antibody (ab62301) at 2.5µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 10 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC46A3 with ab62301 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.

References

ab62301 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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