Overview

  • Product nameAnti-SLC46A3 antibody
  • Description
    Rabbit polyclonal to SLC46A3
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human SLC46A3 (MKILFVEPAI FLSAFAMTLT GPLTTQYVYR RIWEETGNYT FSSDSNISEC)

  • Positive control
    • Jurkat cell lysate. Human kidney.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceSLC46A3 (Solute carrier family 46 member 3) is a member of the major facilitator superfamily and SLC46A family. Its function remains unknown.
  • Cellular localizationMembrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • FKSG 16 antibody
    • FKSG16 antibody
    • FLJ42613 antibody
    • OTTHUMP00000018184 antibody
    • RP11 97E23.2 antibody
    • Solute carrier family 46 member 3 antibody
    see all

Anti-SLC46A3 antibody images

  • All lanes : SLC46A3 antibody (ab62301) at 2.5µg/ml (in 5% skim milk / PBS buffer)

    Lane 1 : Molecular weight marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 10 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SLC46A3 with ab62301 at 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-SLC46A3 antibody (ab62301)

ab62301 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62301.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"